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EDN

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After activation eosinophil granulocytes are segregating the cationic glycoprotein EDN (eosinophil derived neurotoxin). This 18-21 kDa single stranded glycosylated protein is also known as EPX (eosinophil protein X). Together with ECP (eosinophil cationic protein) EDN belongs to the ribonuclease superfamily (Lit. 1-3). However EDN has a 100-fold increased ribonuclease activity. It is neurotoxic but not cytotoxic (Lit 4, 5). The activation of eosinophil granulocytes is important during inflammatory processes in allergic reactions. Thus EDN is a marker for eosinophile activation and degranulation.

The measurement of EDN in stool allows the detection of clinical or subclinical chronic inflammation in the gut. In patients with colitis ulcerosa and/or morbus crohn, the measurement of EDN gives information on the activity of the disease and the prediction of a rezidiv.

 

Indications

  • Differentiation of food allergy and food intolerance
  • Monitoring of an elimination diet
  • Inflammatory processes in the gut
  • Intestinal parasitosis

 

 

Principle of the method

The EDN-ELISA test determines human EDN according to the “sandwich”-principle. EDN in sample, standard and controls binds to monoclonal antibodies, which are coated to the microtiterplate. After a washing step a peroxidase labeled polyclonal antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm in a microtiterplate reader. The EDN concentration can be calculated from the standard curve.